Genome-Wide Analysis of the Indispensable Role of Non-structural Proteins in the Replication of SARS-CoV-2

Jun 1, 2022·
Yunyun Jin
Muzi Ouyang (Mia)
Muzi Ouyang (Mia)
,
Ting Yu
,
Jiaxin Zhuang
,
Wenhao Wang
,
Xue Liu
,
Fangfang Duan
,
Deyin Guo
,
Xiaoxue Peng
,
Ji-An Pan
· 0 min read
DOI
Abstract
Understanding the process of replication and transcription of SARS-CoV-2 is essential for antiviral strategy development. The replicase polyprotein is indispensable for viral replication. However, whether all nsps derived from the replicase polyprotein of SARS-CoV-2 are indispensable is not fully understood. In this study, we utilized the SARS-CoV-2 replicon as the system to investigate the role of each nsp in viral replication. We found that except for nsp16, all the nsp deletions drastically impair the replication of the replicon, and nsp14 could recover the replication deficiency caused by its deletion in the viral replicon. Due to the unsuccessful expressions of nsp1, nsp3, and nsp16, we could not draw a conclusion about their in trans-rescue functions. Our study provided a new angle to understand the role of each nsp in viral replication and transcription, helping the evaluation of nsps as the target for antiviral drug development.
Type
Publication
Frontiers in Microbiology, 13, 907422
Status
Peer-reviewed Open access
publications
SARS-CoV-2 Viral Replication Functional Genomics
Muzi Ouyang (Mia)
Authors
Muzi Ouyang (Mia)
PhD Candidate

I am Muzi Ouyang (欧阳木子) — I go by Mia. I am a PhD candidate in Chemistry at Tsinghua University, advised by Prof. ADVISOR NAME. My research is on YOUR TOPIC — ONE SENTENCE ON WHAT YOU FOUND OR BUILT.

My background spans animal science, basic medicine and chemistry, so I work comfortably at the interface between them. (Rewrite or delete this line — but a deliberate framing of an interdisciplinary path reads as a strength, whereas leaving it unexplained invites the question.)

I expect to defend in June 2027 and am seeking postdoctoral positions starting in late 2027. If my work overlaps with your group’s, I’d be glad to hear from you — my CV is linked above. Write to me at mumumia.1009@gmail.com, or oymzmia@163.com if you are corresponding from within China.